The measurement range extends from 1 x 10e4 to 1 x 10e7 cellsml the optimal range is 1 x 10e5 to 4 x 10e6 cellsml, which is broader than that of a. Col05332 simple and accurate monitoring of adipogenesis. On 11 june 2008, applera corporation now known as applied biosystems inc. I count them anyways and proceed to validate my results with the tc20 automated cell counter by biorad and confirm that they show similaridentical percentage of live vs dead cells. The number of cells in each well was counted using an automated cell counter countess, invitrogen, carlsbad, ca and the total cellbound radioactivity tb was measured in a gamma counter wizard 2480, perkin elmer, ma. To purchase, call invitrogen customer service at 1 800 9556288 for academic and industrial customers or 1 888 5848929 for government customers. Invitrogen countess ii fl automated cell counter countess ii. Invitrogen thermo fisher countess ii automated cell counter. Countess ii automated cell counter is a benchtop assay platform equipped with stateoftheart optics, full autofocus, and image analysis software for rapid assessment of cell s in suspension. The invitrogen countess ii fl automated cell counter provides fast, accurate measurements of cell viability and concentration that can be performed both before and after isolation and activation, saving time and precious cells for future experiments. Performing a simple brightfield count on the countess ii fl automated cell counter. Unbound radioactivity was removed and the dishes were rinsed three times with icecold pbs and detached with trypsin. This location in carlsbad is now the headquarters of life technologies, but was originally the headquarters of invitrogen. Prior to this merger, invitrogen acquired biosource international and quatum dot corporation in 2005.
Our global team of more than 75,000 colleagues delivers an unrivaled combination of innovative technologies, purchasing convenience and pharmaceutical services through our industryleading brands, including thermo scientific, applied biosystems, invitrogen, fisher. Countess ii automated cell counter from thermo fisher scientific. Genelab houses an accuri c6 flow cytometer, nexcelom biosciences cellometer vision cell profiler, and an invitrogen countess automated cell counter. Whether you are labeling proteins and antibodies for immunofluorescence, nucleic acids for in situ hybridization, or lipids for membrane studies, this.
May 26, 2009 the countess automated cell counter from invitrogen is a benchtop automated cell counter. The countess ii fl automated cell counter is a benchtop assay platform equipped with stateoftheart optics, full autofocus, and image analysis software for rapid assessment of cells in suspension. Its image quality allows us to define easily cells of interest and its software is very precise. Get the inside scoop on jobs, salaries, top office locations, and ceo insights.
With threechannel flexibilitybrightfield and two optional fluorescence channelsresearchers can count cells, monitor fluorescent protein expression, evaluate ap. Invitrogen countess ii automated cell counter countess ii. The countess ii fl automated cell counter uses trypan blue staining combined with an autofocus mechanism and sophisticated image analysis algorithm to obtain accurate cell and viability counts. The countess automated cell counter from invitrogen is a benchtop automated cell counter. Countess ii automated cell counter is a benchtop assay platform equipped with stateoftheart optics, full autofocus, and image analysis software for rapid assessment of cells in suspension. The invitrogen countess automated cell counter uses trypan blue stain to stain cells for counting and viability studies. Cell sizes, mean diameter, total cell concentration, graphical data presentation and images are some of the parameters that can be determined using the countess. The countess ii automated cell counter is a benchtop assay platform equipped with stateoftheart optics, full autofocus, and image analysis software for rapid assessment of cells in suspension. Invitrogen countess ii fl automated cell counter can be used. I count them anyways and proceed to validate my results with the tc20 automated cell counter by biorad and confirm that they show similaridentical percentage of. The company enables its customers to make the world healthier, cleaner and safer by providing analytical instruments.
A wide variety of prokaryotic cell can be counted within a very short time, usually less than a minute per sample. Mar 17, 2020 countess ii automated cell counter from thermo fisher scientific description the new countess ii automated cell counter is a benchtop assay platform equipped with stateoftheart optics, full autofocus, and image analysis software for rapid assessment of cells in suspension. Invitrogens product family includes many of the most widely recommended names in the industry, including invitrogen, molecular probes, gibco, biosource and dynal biotech. The countess automated cell counter is a benchtop counter designed to measure cell count and viability live, dead, and total cells accurately and precisely, using the standard trypan blue technique. Mix 10 l of sample with 10 l of trypan blue and pipet into a disposable countess chamber slide. Mix 10l of sample with 10l of trypan blue and pipet into a disposable countess chamber slide.
Countess automated cell counter from invitrogen biocompare. Uncover why invitrogen is the best company for you. Our global team of more than 75,000 colleagues delivers an unrivaled combination of innovative technologies, purchasing convenience and pharmaceutical services through our industryleading brands, including thermo scientific, applied biosystems, invitrogen, fisher scientific, unity lab services and patheon. Invitrogen corporation 1600 faraday avenue carlsbad, ca 92008 t. With threechannel flexibilitybrightfield and two optional fluorescence channelsresearchers can count cells, monitor fluorescent protein. Welcome to the labeling chemistry selection tool featuring invitrogen molecular probes reagents and labeling technologies. Weve tried surface antigen detection, for instance such as anticd antibodies but with poor detection. More than 4,800 employees work for invitrogen and we conduct business in over 70 countries.
Col05332 simple and accurate monitoring of adipogenesis app. It can perform cell counts as well as viability and cell calculations. C6 flow cytometer the accuri c6 flow cytometer, cflow software and workstation pc supply all the capabilities of a fullfeatured flow cytometer. In november 2008, life technologies was created by the combination of invitrogen corporation and applied biosystems inc. Applied biosystems whether youre looking for everyday productivity or cutting edge technology, thermo scientific, applied biosystems, invitrogen and gibco reagents, consumables and instruments will deliver the consistent results that you need every time.
Invitrogen countess cell counting chamber slides designed for use with countess automated cell counter. Invitrogen corporation life technologies corporation. Cells of interest can be stained with a viability dye, such as invitrogen sytox red dead cell stain, as. The product line includes various subbrands of biotechnology products, such as machines and consumables for polymerase chain reaction, reverse transcription, cloning, culturing, stem cell production, cell therapy, regenerative medicine, immunotherapy, transfection, dnarna purification, diagnostic tests. This simple approach can be used to monitor differentiation for. The countess automated cell counter offers an intuitive user interface, and provides the option to save and print cell count data using the countess software download from vitrogen.
Apoptosis in addition to cell viability, knowing how many cells are dead or dying are key pieces of information that can be determined quickly with the countess ii fl instrument. Detection of fluorescently immunolabeled cells using. Drawbacks are the price and that it needs its own slides for counting that cost a lot. With threechannel flexibilitybrightfield and two optional fluorescence channelsresearchers can count cells, monitor fluorescent protein expression, evaluate apoptosis, and measure cell viability. The countess automated cell counter uses stateoftheart optics and image analysis to automate cell counting. The case is a complement to sm209a and sm209b, which highlight the various growth and acquisition strategies the company undertook over the previous 10 years. D espite the potency and durability of current antiretroviral arv regimens, sustained drug exposure continues to be the main predictor of efficacy in hiv1 hiv treatment. The blockit rnai designer is such an effective tool for the design of stealth rnai sirna that if you order the three best stealth rnai sirna sequences designed by the blockit rnai designer, we guarantee that two of them will give greater than 70% knockdown of mrna, given that the transfection efficiency in your experiment is at least 80%. The countess ii fl automated cell counter works in 3 simple steps. Invitrogen is one of several brands under the thermo fisher scientific corporation. Jun 15, 2015 unbound radioactivity was removed and the dishes were rinsed three times with icecold pbs and detached with trypsin. The countess has a touch screen display and allows data to be transferred via usb drive to a computer for data access and storage. Invitrogen healthcare sector ta a private equity firm. Our unique and innovative technologies help the world discover and produce better ways to heal, feed, protect.
We provide products and services to pharmaceutical and biotechnology companies, as well as academic and government research institutions. Used invitrogen countess automated cell counter 6002701. Download the latest countess iiii fl software version to your desktop. This short case discusses the process of life technologies acquisition by thermo fisher in 20 and 2014. Countess ii fl automated cell counter thermo fisher.
Accurateeliminate the subjectivity of manual cell counting and usertouser variability. The invitrogen countess ii fl automated cell counter offers brightfield counting and two optional, user changeable fluorescence channels. Invitrogen thermo fisher countess ii automated cell. Invitrogen countess ii fl automated cell counter countess. See who you know at invitrogen, leverage your professional network, and get hired. Invitrogen countess ii automated cell counter fisher.
Cell counter countess ii stands out for its ease of use. The countess ii fl system is a benchtop cell counter with fluorescence detection capabilities, allowing for quick and accurate monitoring of adipogenesis when used with the hcs lipidtox neutral lipid stains. Refurbished invitrogen countess automated cell counter. Pet imaging of glioblastoma multiforme egfr expression for. The countess ii automated cell counter works in 3 simple steps. The countess software allows cells to be gated based on size, brightness, and circularity, allowing easy inclusion or. Invitrogen countess ii fl automated cell counter starter. Invitrogen offers 35,000 unique products and services to support disease research, drug discovery and commercial bioproduction. Whether youre looking for everyday productivity or cutting edge technology, thermo scientific, applied biosystems, invitrogen and gibco reagents, consumables and instruments will deliver the consistent results that you need every time. Apr 01, 2015 d espite the potency and durability of current antiretroviral arv regimens, sustained drug exposure continues to be the main predictor of efficacy in hiv1 hiv treatment.
Countess automated cell counter from thermo scientific. Used invitrogen countess automated cell counter 8000366. Accurateuse the countess ii automated cell counter to eliminate the subjectivity of manual cell counting and usertouser variability. But nothing counts like countess ii automated cell counters. Quickly find the best reactive dye or label for your experiment. Invitrogens research kits simplify and improve gene cloning, gene expression and gene analysis techniques. Invitrogen countess ii fl automated cell counter from. It is a global supplier of instrumentbased systems, consumables,2 software, and services to academic research, the life science industry and commercial markets.
512 712 144 543 12 329 1268 725 1230 311 1021 1139 998 401 123 520 122 704 795 703 1533 15 251 1035 800 1292 268 1501 589 979 1051 59 1281 679 1532 1227 403 268 58 1020 491 294 97 1018 1238 1044 1436 612